Primary Human Kidney Cortical Cells (PHKCC) are specialized microvascular cells that make up the innermost layer of the glomerular filtration barrier. They are separated from podocytes by a thin layer of glomerular basement membrane. These cells exhibit cobblestone morphology at confluence and present fenestrations specific of this cell type. The PHKCC are obtained after cortex digestion, and single glomerular isolation. Glomeruli are further digested and then PHKCC are isolated by CD31 positive selection as a pure population. The PHKCC express factor VIII-related antigen, angiotensin converting enzyme activity, and endocytosed acetylated low-density lipoproteins. They are also characterized by the presence of intracellular. Weibel-Palade bodies and caveolae and microvillous projections on the luminal surface.
The cells are evaluated for the expression of endothelial specific markers (e.g. CD31, vWF and Ve-Cadherin) by Immunohistochemistry and Western Blot. In addition, assessment of cellular morphology will be performed by comparing them to a known standard. PHKCC can be expanded between 5 to 8 passages, while maintaining their particular characteristics. PHKCC are terminally differentiated cells and present no significant differentiation capacity. The PHKCC are for research use only and not approved for human or animal use, nor for in vitro diagnostic testing.
PHKCC can be used for drug and toxicity screening, drug transporter research, tissue engineering as well as general research. They are an excellent source for FACS selection of more homogenous populations of multiple renal cell types.
The cells are isolated using animal-free conditions and enzymes isolated from bacteria.