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Primary Human Tracheal Epithelial Cells

Primary Human Tracheal Epithelial Cells (PHTEC) can be used as a source for repeated differentiated cell stocks useful for any analysis procedure typically performed with freshly isolated cells derived from ex vivo tissue. Given the cell composition of ciliated, non-ciliated, goblet and basal cells, PHTEC are a good source of a mixed cell population for use in an in vitro model for studying the function and pathophysiology of the respiratory epithelium. PHTEC are a perfect source for single-cell analysis from individual donors. PHTEC allow a researcher to perform FACS analysis of the diverse population of epithelial cells found within the tracheal cell composition. PHTEC also allow for a representative sample of RNA/DNA/Protein from the epithelial cell mix which is representative of individual donors.



Product Code: ax134476 (500,000 cells) Categories: .

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Features & Benefits

Primary Human Human Tracheal Epithelial Cells (PHTEC) are isolated from the trachea of healthy human donors but do not contain epithelial cells from the primary bronchus. Each vial of the PHTEC contains the complete tracheal cell composition consisting of differentiated cells, including ciliated and mucin producing cells as well as basal progenitor cells. PHTECs have been characterized and Air/Liquid Interface culture validated by immunofluorescence using protein markers for Cilia (acetylated tubulin), mucin (MUC5AC), tight junctions (Zonula Occludens-1), and basal cells (cytokeratin-5). These cells are guaranteed to have minimum of at least 30 population doublings from passage number zero (P0) following the instructions and conditions provided by Axol. 

Different passages are available, please enquire for latest availability. Researchers wanting a representative sample of human airway epithelia should choose P0 cells over P1. Cells from a P1 or P2 stock from other manufacturers lack the diversity of the original epithelial cell mixture that comes from freshly isolated P0 cells from individual donors. 

PHTEC cells can be grown with any method amenable to expansion of primary airway epithelia, so if a researcher has a particular need for different culture conditions (e.g. animal-free media), then this is an excellent choice of cell stock. Our cells are isolated using animal-free conditions and enzymes isolated from bacteria.

PHTEC are for research use only and not approved for human or animal use, nor for in vitro diagnostic testing. 

Product Specification

Donor age 62
Donor disease N/A
Donor mutation N/A
Cell subtype Primary
Cell source Human Lung
Quantity 500,000 cells/vial
Donor gender Male
HLA serotype N/A
Karyotype Normal
Genetic modification N/A
Growth properties Adherent
Shipping conditions Dry ice
Storage conditions Liquid nitrogen