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Recombinant Human EGF

For administrative reasons ax0047X has changed to ax0048
Product Code: ax0048 Categories: , .

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Description

Recombinant Human EGF is a xeno-free growth supplement necessary for promoting cellular proliferation and expansion of progenitor cells. It is provided as a lyophilized powder.

Product Specification

Size 100 µg
Purity Greater than 95% by SDS-PAGE
Shipping conditions Blue ice
Storage conditions -20°C
Source Produced in the endosperm tissue of barley grain (Hordeum vulgare)
Endotoxin level <0.005 ng per µg of recombinant protein (0.05 EU/µg)
Reconstitution Centrifuge the vial before opening. Reconstitute the lyophilized protein in sterile phosphate-buffered saline (PBS) + 0.1% Human Serum Albumin (HSA). The concentration of the reconstituted protein solution should be >100 µg/mL.
Stability The lyophilized protein should be stored at -20°C. The reconstituted protein solution should be used immediately or stored in working aliquots at -20°C. Avoid repeated freeze-thaw cycles.
Xeno free Yes
Usage notes The optimal concentration should be determined for specific applications and cell lines. For use with Axol Neural Expansion-XF Medium (ax0030-500), supplement the medium with 20 ng/mL Recombinant Human EGF.

Frequently Asked Questions

We recommend adding the growth factors EGF and FGF2 fresh each time Neural Expansion-XF Medium is used.

Please do not passage the NSCs more than 3 times as the cells will transition to gliogenic state over longer term culture. We found that the cells treated with growth factors (EGF and FGF2) gradually adopt a "flat" morphology, however, when the growth factors are removed, they revert back to spindle-like morphology. This is completely normal. It is best to withdraw the growth factors (EGF and FGF2) first (at least a couple of days) before differentiation using neural differentiation medium.