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Description
Recombinant Human FGF2 is a xeno-free growth supplement necessary for promoting cellular proliferation and expansion of progenitor cells. It is provided as a lyophilized powder.
Product Specification
Size
100 µg
Purity
Greater than 95% by SDS-PAGE
Shipping conditions
Blue ice
Storage conditions
-20°C
Source
Produced in the endosperm tissue of barley grain (Hordeum vulgare)
Endotoxin level
<0.005 ng per µg of recombinant protein (0.05 EU/µg)
Reconstitution
Centrifuge the vial before opening. Reconstitute the lyophilized protein in sterile phosphate-buffered saline (PBS) + 0.1% Human Serum Albumin (HSA). The concentration of the reconstituted protein solution should be >100 µg/mL.
Stability
The lyophilized protein should be stored at -20°C. The reconstituted protein solution should be used immediately or stored in working aliquots at -20°C. Avoid repeated freeze-thaw cycles.
Xeno free
Yes
Usage notes
The optimal concentration should be determined for specific applications and cell lines. For use with Axol Neural Expansion-XF Medium (ax0030-500), supplement the medium with 20 ng/mL Recombinant Human FGF2.
Frequently Asked Questions
Can the Neural Expansion-XF Medium be frozen once supplemented?
We recommend adding the growth factors EGF and FGF2 fresh each time Neural Expansion-XF Medium is used.
How long can I expand the NSCs before differentiation?
Axol do not recommend the expansion of NSCs prior to differentiation. For best results, thaw the frozen NSC and then begin the differentiation process 24 hours later.
Recombinant Human FGF2 is a xeno-free growth supplement necessary for promoting cellular proliferation and expansion of progenitor cells. It is provided as a lyophilized powder.
Size
100 µg
Purity
Greater than 95% by SDS-PAGE
Shipping conditions
Blue ice
Storage conditions
-20°C
Source
Produced in the endosperm tissue of barley grain (Hordeum vulgare)
Endotoxin level
<0.005 ng per µg of recombinant protein (0.05 EU/µg)
Reconstitution
Centrifuge the vial before opening. Reconstitute the lyophilized protein in sterile phosphate-buffered saline (PBS) + 0.1% Human Serum Albumin (HSA). The concentration of the reconstituted protein solution should be >100 µg/mL.
Stability
The lyophilized protein should be stored at -20°C. The reconstituted protein solution should be used immediately or stored in working aliquots at -20°C. Avoid repeated freeze-thaw cycles.
Xeno free
Yes
Usage notes
The optimal concentration should be determined for specific applications and cell lines. For use with Axol Neural Expansion-XF Medium (ax0030-500), supplement the medium with 20 ng/mL Recombinant Human FGF2.
How to Order
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