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Description
Recombinant Human FGF2 is a xeno-free growth supplement necessary for promoting cellular proliferation and expansion of progenitor cells. It is provided as a lyophilized powder.
Product Specification
Size
100 µg
Purity
Greater than 95% by SDS-PAGE
Shipping conditions
Blue ice
Storage conditions
-20°C
Source
Produced in the endosperm tissue of barley grain (Hordeum vulgare)
Endotoxin level
<0.005 ng per µg of recombinant protein (0.05 EU/µg)
Reconstitution
Centrifuge the vial before opening. Reconstitute the lyophilized protein in sterile phosphate-buffered saline (PBS) + 0.1% Human Serum Albumin (HSA). The concentration of the reconstituted protein solution should be >100 µg/mL.
Stability
The lyophilized protein should be stored at -20°C. The reconstituted protein solution should be used immediately or stored in working aliquots at -20°C. Avoid repeated freeze-thaw cycles.
Xeno free
Yes
Usage notes
The optimal concentration should be determined for specific applications and cell lines. For use with Axol Neural Expansion-XF Medium (ax0030-500), supplement the medium with 20 ng/mL Recombinant Human FGF2.
Frequently Asked Questions
How long do I need to expand the cells before I start differentiating the cells?
Each line will need to be assessed to understand it's growth Profile, lines vary however you should aim to passage no more than 2 times
Always allow the cells to recover from mitogen treatment for at least a couple of days before going into differentiation. You do not want to differentiate when cells appear flat. Let the cells recover from the flat morphology and assume NSC like morphology before changing media to diff-XF.
Recombinant Human FGF2 is a xeno-free growth supplement necessary for promoting cellular proliferation and expansion of progenitor cells. It is provided as a lyophilized powder.
Size
100 µg
Purity
Greater than 95% by SDS-PAGE
Shipping conditions
Blue ice
Storage conditions
-20°C
Source
Produced in the endosperm tissue of barley grain (Hordeum vulgare)
Endotoxin level
<0.005 ng per µg of recombinant protein (0.05 EU/µg)
Reconstitution
Centrifuge the vial before opening. Reconstitute the lyophilized protein in sterile phosphate-buffered saline (PBS) + 0.1% Human Serum Albumin (HSA). The concentration of the reconstituted protein solution should be >100 µg/mL.
Stability
The lyophilized protein should be stored at -20°C. The reconstituted protein solution should be used immediately or stored in working aliquots at -20°C. Avoid repeated freeze-thaw cycles.
Xeno free
Yes
Usage notes
The optimal concentration should be determined for specific applications and cell lines. For use with Axol Neural Expansion-XF Medium (ax0030-500), supplement the medium with 20 ng/mL Recombinant Human FGF2.
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