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Description
The medium promotes the viability and maturation of sensory neurons and permits expression of the sodium channels Nav1.7 and Nav1.8. The medium requires supplementing with Glial-Derived Neurotrophic Factor (GDNF; 25 ng/mL), Nerve Growth Factor (NGF; 25 ng/mL), Brain-Derived Neurotrophic Factor (BDNF; 10 ng/mL) and Neurotrophin-3 (NT-3; 10 ng/mL) that are not supplied.
Product Specification
Size
250 mL
Shipping conditions
Dry ice
Storage conditions
-80°C
Sterility
Sterile
Usage notes
The medium requires supplementing with Glial-Derived Neurotrophic Factor (GDNF), Nerve Growth Factor (NGF), Brain-Derived Neurotrophic Factor (BDNF) and Neurotrophin-3 (NT-3)
Frequently Asked Questions
What percentage of Sesory neurons express TRPV1?
70-80% sensory neurons express TRPV1 in our immunostaining.
Can I expand iPSC-derived sensory neuron progenitors?
No, This product is a late stage progenitor and are not proliferative. Also, they need to be treated with an anti-mitotic at day 3 so this will halt any remaining proliferative potential
How long can iPSC-derived sensory neuron progenitors be kept in culture?
Sensory neurons can be kept in continuous culture for over 10 months.
* Special culture method is not required. The Normal culture protocol can be adhered to.
The medium promotes the viability and maturation of sensory neurons and permits expression of the sodium channels Nav1.7 and Nav1.8. The medium requires supplementing with Glial-Derived Neurotrophic Factor (GDNF; 25 ng/mL), Nerve Growth Factor (NGF; 25 ng/mL), Brain-Derived Neurotrophic Factor (BDNF; 10 ng/mL) and Neurotrophin-3 (NT-3; 10 ng/mL) that are not supplied.
The medium requires supplementing with Glial-Derived Neurotrophic Factor (GDNF), Nerve Growth Factor (NGF), Brain-Derived Neurotrophic Factor (BDNF) and Neurotrophin-3 (NT-3)
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