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Sensory Neuron Maintenance Medium

Sensory Neuron Maintenance Medium for the maintenance of sensory neuron progenitors and derived neurons. 

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Description

The medium promotes the viability and maturation of sensory neurons and permits expression of the sodium channels Nav1.7 and Nav1.8. The medium requires supplementing with Glial-Derived Neurotrophic Factor (GDNF; 25 ng/mL), Nerve Growth Factor (NGF; 25 ng/mL), Brain-Derived Neurotrophic Factor (BDNF; 10 ng/mL) and Neurotrophin-3 (NT-3; 10 ng/mL) that are not supplied. 

Product Specification

Size 250 mL
Shipping conditions Dry ice
Storage conditions -80°C
Sterility Sterile
Usage notes The medium requires supplementing with Glial-Derived Neurotrophic Factor (GDNF), Nerve Growth Factor (NGF), Brain-Derived Neurotrophic Factor (BDNF) and Neurotrophin-3 (NT-3)

Frequently Asked Questions

For terminally differentiated cultures of sensory neurons, you can change medium every 2 to 3 days.

Yes.

No, these cells would need to be treated with mitomycin C at day 3 which would halt any remaining proliferative potential.

After 5 weeks of differentiation, 70-80% of terminally differentiated sensory neurons express TRPV1 as validated with ICC.

Sensory neurons can be kept in continuous culture for over 10 months. Special culture method is NOT required. The standard culture protocol and Sensory neuron maintenance medium can be used for supporting the long term culture.

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