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Description
The medium promotes the viability and maturation of sensory neurons and permits expression of the sodium channels Nav1.7 and Nav1.8. The medium requires supplementing with Glial-Derived Neurotrophic Factor (GDNF; 25 ng/mL), Nerve Growth Factor (NGF; 25 ng/mL), Brain-Derived Neurotrophic Factor (BDNF; 10 ng/mL) and Neurotrophin-3 (NT-3; 10 ng/mL) that are not supplied.
Product Specification
Size
250 mL
Shipping conditions
Dry ice
Storage conditions
-80°C
Sterility
Sterile
Usage notes
The medium requires supplementing with Glial-Derived Neurotrophic Factor (GDNF), Nerve Growth Factor (NGF), Brain-Derived Neurotrophic Factor (BDNF) and Neurotrophin-3 (NT-3)
Frequently Asked Questions
How often do I have to change maintenance medium for sensory neurons?
For terminally differentiated cultures of sensory neurons, you can change medium every 2 to 3 days.
Shall I Filtration Sterilize the medium after Mitomycin C addition before treating the cells?
Yes.
Can I expand the iPSC-derived sensory neuron progenitors?
No, these cells would need to be treated with mitomycin C at day 3 which would halt any remaining proliferative potential.
What percentage of sensory neurons would express TRPV1 in my culture?
After 5 weeks of differentiation, 70-80% of terminally differentiated sensory neurons express TRPV1 as validated with ICC.
How long can iPSC-derived sensory neuron progenitors be kept in culture?
Sensory neurons can be kept in continuous culture for over 10 months.
Special culture method is NOT required. The standard culture protocol and Sensory neuron maintenance medium can be used for supporting the long term culture.
The medium promotes the viability and maturation of sensory neurons and permits expression of the sodium channels Nav1.7 and Nav1.8. The medium requires supplementing with Glial-Derived Neurotrophic Factor (GDNF; 25 ng/mL), Nerve Growth Factor (NGF; 25 ng/mL), Brain-Derived Neurotrophic Factor (BDNF; 10 ng/mL) and Neurotrophin-3 (NT-3; 10 ng/mL) that are not supplied.
The medium requires supplementing with Glial-Derived Neurotrophic Factor (GDNF), Nerve Growth Factor (NGF), Brain-Derived Neurotrophic Factor (BDNF) and Neurotrophin-3 (NT-3)
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