Figure 1. Calcium Imaging of the iPSC-sensory neurons' responses to 1 μM Capsaicin when cultured with and without Maximizer. When cultured with Sensory Neuron Maturation Maximizer Supplement, sensory neurons respond to low concentrations of Capsaicin indicating the presence of functional TRPV1 channels.
A. Average deltaF/Fo values for the unsupplemented and Maximizer-supplemented sensory neurons treated with vehicle control and 1 μM capsaicin on Day 20 of culture.
B & C. Pie Chart and Table showing capsaicin threshold concentrations for sensory neurons treated with and without Maximizer. 91% of Regions-Of-Interest (ROIs) show a response to the lowest concentration of capsaicin tested (1 μM) compared to only 19% of control cells and while 99% of the ROIs of treated sensory neurons respond to the highest concentration of capsaicin (100 μM), 57% of non-treated sensory neurons remain non-responsive.
This shows that even by Day 20, the Maximizer-treated sensory neurons show enhanced capsaicin responses compared to non-treated.