ReadySet: 12 mL
SureBond: 90 uL
Striatal Neuron Medium: 85 mL
Below we laid out the calculations:
Assuming you'd like to plate the cells at our recommended density - 35,000 cells per square centimetre, you would have 43 square centimetres (or 4.5 wells of a 6-well plate) of cells.
For ReadySet coating, we recommend 250 uL per square centimetre. So, you'd need 11 mL of ReadySet per vial of cells (250 uL x 43 cm2)
For SureBond top coating, we recommend adding 120 uL in 6 mL PBS; and then coat 100 uL of diluted SureBond per square centimetre. So, you'd need at least 86 uL of SureBond (in 4.3 mL of PBS) per vial of cells.
Assuming you'd use 6-well plates to start with your experiments, each well would require 2 mL medium.
For plating 1 vial of cells (19 mL total):
Spin down: 10 mL
Then, 2 mL for each well x 4.5 wells = 9 mL
Medium A for 1 vial of the cells (40 mL total):
Day 1 - complete medium change - 2 mL for each well x 4.5 wells = 9 mL
Day 4, 7, 10, 13 and 16 - 2/3 medium change (1.33 mL) - 6.7 mL total for each well x 4.5 wells = 30.2 mL
Medium B for 1 vial of the cells (31 mL total):
Day 19, 22, 25, 28 and 31 - 2/3 medium change - 6.7 mL x 4.5 wells = 30.2 mL
Hence, you'd need 81 mL of medium per vial of cells to differentiate and mature the cells to Day 33 for assaying.