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SureBond

Coating solution that provides an optimal surface when expanding Human iPSC-Derived Neural Stem Cells.

Product Code: ax0041-kit Categories: .

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Description

SureBond is a coating solution that provides an optimal surface when expanding Human iPSC-Derived Neural Stem Cells. It permits neural cells to be passaged with Unlock (ax0044).

Optimized Coating Solution for Neural Stem Cells and Neurons

SureBond coating solution provides an optimal surface for culturing iPSC-derived neural stem cells and neurons.
It should be used when expanding neural stem cells and when passaging of the cells with Unlock is required. 

Product Specification

Size 3 x 120 μL vials
Concentration 50x
Shipping conditions Dry ice
Storage conditions -80°C
Usage notes Centrifuge the vial before opening.

Frequently Asked Questions

Always plate onto Surebond (ax0041) if you intend to detach the cells for continued expansion

SureBond-XF: Xeno-free coating for endpoint assays; SureBond: coating reagent needed when passaging is required; Surebond & Readyset: coating reagent kit for endpoint assays on glass.

Please make sure you change medium gently and avoid adding the medium from one side of the wells throughout the 5-6 weeks of culture. If the cells start to peel from the corners, it can be repaired by adding Surebond (ax0041) into your standard feeding media. Usually, we use 120 uL Surebond in 12 mL medium for a few days until the layer re-attach. This can be applied no matter what coating has been used.

For best results, use Surebond + Readyset for final plating.

References

  • Odawara A, Katoh H, Matsuda N, et al., Induction of long-term potentiation and depression phenomena in human induced pluripotent stem cell-derived cortical neurons. Biochemical and Biophysical Research Communications (2015)
  • Odawara A, Katoh H, Matsuda N, et al., Physiological maturation and drug responses of human induced pluripotent stem cell-derived cortical neuronal networks in long-term culture. Scientific Reports (2016)