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Description
SureBond-XF is a Xeno-free coating solution optimized for long-term or low density culture of human iPSC-derived neural stem cells, neurons and astrocytes
Axol SureBond-XF provides a defined, xeno-free surface specifically designed to promote optimal growth and attachment of human neural cells. After coating, and a short 4-hour incubation period (at 37oC), your cell culture flask or plate would be ready to receive your cells allowing you to get on with your research and saving your valuable time.
Advantages
200X concentrate, 1 mL stock SureBond-XF is enough for coating 1000 square centimetre of culture surface area
Supports low density cultures
Xeno-free coating solution
Short coating procedure (4 hours)
Axol Human iPSC-derived Neural Stem Cells (ax0015) were expanded on vessels coated with SureBond-XF and passaged using Unlock-XF (ax0054). Post-passage the neural stem cells were left to spontaneously differentiate in Neural Maintenance-XF Medium(ax0032-500).
Tuj1DAPI SureBond-XF supports low density cultures
Human Neural Stem Cells (ax0016) were plated onto SureBond-XF coated culture vessels at low density.
Nestin FoxG1 Human Neural Stem Cells (ax0015) cultured on SureBond-XF coated culture vessels.
Pax6NestinDAPI
Human Neural Stem Cells (ax0015) cultured on SureBond-XF coated culture vessels.
Product Specification
Size
1 mL
Concentration
200X
Storage conditions
4°C
Xeno free
Yes
Sterility
Sterile
Frequently Asked Questions
I am not sure what coating I should use for iPSC-derived neural stem cells?
We recommend SureBond-XF: Xeno-free coating reagent (Ax0053) in combination with Poly-D-Lysine (Sigma Aldrich, P7405)
Can I leave SureBond-XF coating solution in the culture vessel for longer than 4 hours?
Yes, SureBond-XF can be used for an overnight coating.
References
Wang W, Wang L, Lu, J, et al., The inhibition of TDP-43 mitochondrial localization blocks its neuronal toxicity. Nature Medicine (2016)
SureBond-XF is a Xeno-free coating solution optimized for long-term or low density culture of human iPSC-derived neural stem cells, neurons and astrocytes
Axol SureBond-XF provides a defined, xeno-free surface specifically designed to promote optimal growth and attachment of human neural cells. After coating, and a short 4-hour incubation period (at 37oC), your cell culture flask or plate would be ready to receive your cells allowing you to get on with your research and saving your valuable time.
Advantages
200X concentrate, 1 mL stock SureBond-XF is enough for coating 1000 square centimetre of culture surface area
Supports low density cultures
Xeno-free coating solution
Short coating procedure (4 hours)
Axol Human iPSC-derived Neural Stem Cells (ax0015) were expanded on vessels coated with SureBond-XF and passaged using Unlock-XF (ax0054). Post-passage the neural stem cells were left to spontaneously differentiate in Neural Maintenance-XF Medium(ax0032-500).
Tuj1DAPI SureBond-XF supports low density cultures
Human Neural Stem Cells (ax0016) were plated onto SureBond-XF coated culture vessels at low density.
Nestin FoxG1 Human Neural Stem Cells (ax0015) cultured on SureBond-XF coated culture vessels.
Pax6NestinDAPI
Human Neural Stem Cells (ax0015) cultured on SureBond-XF coated culture vessels.
Size
1 mL
Concentration
200X
Storage conditions
4°C
Xeno free
Yes
Sterility
Sterile
Read now
Wang W, Wang L, Lu, J, et al., The inhibition of TDP-43 mitochondrial localization blocks its neuronal toxicity. Nature Medicine (2016)
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