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Description
SureBond-XF is a xeno-free coating solution optimized for long-term or low density culture of human iPSC-derived neural stem cells and neurons
Axol SureBond-XF provides a defined, xeno-free surface specifically designed to promote optimal growth and attachment of human neural progenitor cells and neurons. After coating, and a short 4-hour incubation period (at 37oC), your cell culture flask or plate will be ready to receive your neural progenitor cells or neurons allowing you to get on with your research and saving your valuable time.
Axol Human iPSC-Derived Neural Stem Cells (ax0015) were expanded on vessels coated with SureBond-XF and passaged using Unlock-XF (ax0044-XF). Post-passage the neural progenitors were left to spontaneously differentiate in Neural Maintenance-XF Medium(ax0032-500).
SureBond-XF supports low density cultures
Human Neural Stem Cells (ax0016) were plated onto SureBond-XF coated culture vessels at low density. The cells were stained for Tuj1 expression and counterstained using DAPI.
Human Neural Progenitors Cultured on Sure Bond-XF form neural rosettes
Human Neural Stem Cells (ax0015) cultured on SureBond-XF coated culture vessels (NestinFoxg1 DAPI).
Human Neural Progenitors Cultured on SureBond-XF express neural progenitor markers
Human Neural Stem Cells (ax0015) cultured on SureBond-XF coated culture vessels express Pax6 and Nestin.
Advantages
Xeno-free coating solution
Short coating procedure (4 hours)
Supports low density cultures
Specifications
Catalog Number: ax0041XF
Concentration: 200X
Format: Liquid
Volume: 1 mL
Sterility: Sterile
Shipping Condition: Blue Ice
Storage: Store at 4ºC and use within 6 months
Applications
Coating cell culture vessels for human neural progenitor cells and cerebral cortical neurons
For administrative reasons ax0041XF has changed to ax0053
Product Specification
Size
1 mL
Storage conditions
4°C
Xeno free
Yes
Sterility
Sterile
Frequently Asked Questions
I am not sure what coating I should use for iPSC-derived neural stem cells?
Surebond-XF - Xeno-free coating reagent needed for endpoint assays on plastic, this is the easiest method of coating, with no wash steps.
Surebond + Readyset - Coating reagent needed for endpoint assays on glass
Surebond- Coating reagent needed when passaging of NSCs is required
References
Wang W, Wang L, Lu, J, et al., The inhibition of TDP-43 mitochondrial localization blocks its neuronal toxicity. Nature Medicine (2016)
SureBond-XF is a xeno-free coating solution optimized for long-term or low density culture of human iPSC-derived neural stem cells and neurons
Axol SureBond-XF provides a defined, xeno-free surface specifically designed to promote optimal growth and attachment of human neural progenitor cells and neurons. After coating, and a short 4-hour incubation period (at 37oC), your cell culture flask or plate will be ready to receive your neural progenitor cells or neurons allowing you to get on with your research and saving your valuable time.
Axol Human iPSC-Derived Neural Stem Cells (ax0015) were expanded on vessels coated with SureBond-XF and passaged using Unlock-XF (ax0044-XF). Post-passage the neural progenitors were left to spontaneously differentiate in Neural Maintenance-XF Medium(ax0032-500).
SureBond-XF supports low density cultures
Human Neural Stem Cells (ax0016) were plated onto SureBond-XF coated culture vessels at low density. The cells were stained for Tuj1 expression and counterstained using DAPI.
Human Neural Progenitors Cultured on Sure Bond-XF form neural rosettes
Human Neural Stem Cells (ax0015) cultured on SureBond-XF coated culture vessels (NestinFoxg1 DAPI).
Human Neural Progenitors Cultured on SureBond-XF express neural progenitor markers
Human Neural Stem Cells (ax0015) cultured on SureBond-XF coated culture vessels express Pax6 and Nestin.
Advantages
Xeno-free coating solution
Short coating procedure (4 hours)
Supports low density cultures
Specifications
Catalog Number: ax0041XF
Concentration: 200X
Format: Liquid
Volume: 1 mL
Sterility: Sterile
Shipping Condition: Blue Ice
Storage: Store at 4ºC and use within 6 months
Applications
Coating cell culture vessels for human neural progenitor cells and cerebral cortical neurons
For administrative reasons ax0041XF has changed to ax0053
Size
1 mL
Storage conditions
4°C
Xeno free
Yes
Sterility
Sterile
Read nowWang W, Wang L, Lu, J, et al., The inhibition of TDP-43 mitochondrial localization blocks its neuronal toxicity. Nature Medicine (2016)
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