• Search

Human iPSC-Derived Astrocytes

Axol Human iPSC-Derived Microglia

iPSC-derived astrocytes

Axol’s Human iPSC-derived Astrocyte Kits include cryopreserved cells, optimised media and supplements. These kits offer researchers a complete culture system that is easy-to-use and promotes long-term cell viability for use in disease modelling and drug discovery applications.

Astrocyte dysfunction has been implicated in a number of neurological conditions such as Alzheimer’s and Parkinson’s diseases, autism, amyotrophic lateral sclerosis (ALS), Rett syndrome and schizophrenia. Axol’s Human iPSC-derived Astrocyte Kits offer a physiologically-relevant tool to study these cells as an isolated population or in co-culture with neurons for complex analysis of the central nervous system (CNS). Human iPSC-derived Astrocyte Progenitors are designed to generate mature astrocytes in under three weeks and iPSC-derived Mature Astrocytes express prototypical phenotype markers such as, glial fibrillary acidic protein (GFAP) and S100b. Our Human iPSC-Derived Astrocyte Progenitor and iPSC-Derived Mature Astrocyte Kits will help researchers gain further insight into the mechanisms that govern CNS development, disease onset and drug response.


iPSC-derived astrocytes

Cat. No. Product Name Donor Starting Material Quantity
ax0083 Human iPSC-Derived Astrocyte Progenitor Kit Male, newborn Cord blood CD34+ cells 1 million cells, medium & supplements
ax0084 Human iPSC-Derived Mature Astrocyte Kit Male, newborn Cord blood CD34+ cells 1 million cells, medium & supplements
ax0081 Human iPSC-Derived Astrocyte Progenitor Kit Female, newborn Cord blood CD34+ cells 1 million cells, medium & supplements
ax0082 Human iPSC-Derived Mature Astrocyte Kit Female, newborn Cord blood CD34+ cells 1 million cells, medium & supplements

Astrocyte culture media and reagents

Cat. No. Product Name Quantity
ax0086 Astrocyte Maintenance Medium 100 mL (basal medium, pluc suplements)

Phase contrast

Phase contrast of Axol Human iPSC-Derived Astrocytes

Phase contrast images of Axol Human iPSC-Derived Astrocyte Progenitors at day 1 (10x) in culture. Human iPSC-derived Astrocytes Progenitors are seeded using Astrocyte Maintenance Medium into a 6-well plate at a density of 100,000 cells/cm2 on Matrigel hESC-Qualified Matrix. After initial seeding the astrocyte progenitors appear flat and elongated, after a further 17 days in culture the morphology becomes more protoplasmic, star-shaped and bushy.


Immunocytochemistry

Immunocytochemistry analysis of Axol Human iPSC-Derived Astrocytes

Axol iPSC-derived Mature Astrocytes express astrocyte-specific markers GFAP and S100B. Low expression of nestin, a marker of progenitor cell phenotype and MAP2, a marker of microtubule growth and neuritogenesis, indicates successful glial differentiation of the iPSCs. Cells seeded on Matrigel at 16,000 cells/well of a 96-well plate. Data provided Susanne Zach, Boehringer Ingelheim.

Axol Mature astrocytes express typical astrocyte markers

Axol iPSC-derived Mature Astrocytes express astrocyte associated markers excitatory amino acid transporter 1 (EAAT1), glutamine synthetase (GS) and ALDH1L1. EAAT1 is active in clearly excitatory glutamate from the extracellular space, preventing neurotoxicity where GS converts glutamate to glutamine. The expression of these markers highlights that Axol’s Human iPSC-derived Astrocytes are physiologically representative of human primary astrocytes. Cells seeded on Matrigel at 16,000 cells/well of a 96-well plate. Data provided Susanne Zach, Boehringer Ingelheim.


Calcium activity

Axol’s Human iPSC-derived Mature Astrocytes were cultured for 7 weeks before measuring the baseline calcium activity.

Human iPSC-derived Mature Astrocytes were loaded with Fluo-4-AM (Thermofisher) calcium dye (1:10 dilution) and incubated for 1 hour at 37oC. The basal activity was recorded without stimulus at a rate of 1 fps. Video clip represents 60 seconds of activity compressed in 5 seconds for visualization. Data kindly provided by Abby Scurfield, Amit K. Chouhan and Prof Gareth Miles (University of St Andrews).