Motor Neuron Progenitors
Axol’s human iPSC-derived motor neuron progenitors have been differentiated using a combination of small molecules to regulate multiple signalling pathways to generate a near pure population of motor neurons progenitors which matured into an enriched population of mature motor neurons.
To support the recovery and maintenance of Axol’s human iPSC-derived motor neuron progenitors, we offer our motor neuron recovery medium which supports cell recovery after thawing and initial cell culture and our motor neuron maintenance medium which promotes the optimal growth and maturation of progenitors to iPSC-derived motor neurons.
We have developed human iPSC-derived motor neuron progenitors from both healthy and patient donors, which can be cultured in vitro to provide a physiologically relevant alternative to conventional animal-derived models for use in proof-of principle drug-screening assays and for modelling motor neuron degeneration.
Human iPSC-derived motor neuron progenitors
|Cat. No.||Product Name||Starting Material||Quantity|
|ax0078||Motor Neuron Progenitors (Healthy)||Fibroblasts (74 yr Male)||≥2 x 106 cells|
|ax0073||Motor Neuron Progenitors (Healthy - sibling (C9ORF72 extension))||Fibroblasts (62 yr Male)||≥2 x 106 cells|
|ax0074||Motor Neuron Progenitors (ALS (C9ORF72 extension)) – coming soon!||Fibroblasts (64 yr Female)||≥2 x 106 cells|
Motor neuron culture media and reagents
|Cat. No.||Product Name||Product Description||Quantity|
|ax0071||Motor Neuron Recovery Medium||Promotes viability of motor neuron progenitors||50 mL|
|ax0072||Motor Neuron Maintenance Medium||For the maintenance of motor neurons||200 mL|
|ax0041||SureBond||Coating solution providing an optimal surface for feeder-free growth & adherence||3 vials of SureBond (120 μL/vial)|
|ax0052||SureBond+ReadySet||Coating reagents required for endpoint assays on glass||3 vials of SureBond (120 μL/vial) and 2 vials of ReadySet (10 mL/vial)|
|ax0044||Unlock||Fully defined & gentle detachment buffer||25 mL|
Phase contrast images show the morphology of human iPSC-derived motor neuron progenitors upon recovery from thawing.
Phase contrast images of Axol Human iPSC-derived motor neuron progenitors at days 1, 3 and 5 after thawing. Human iPSC-derived motor neuron progenitors are seeded using motor neuron recovery medium into T-25 flasks (coated with SureBond) at a density of 100,000 cells/cm2. During initial recovery phase the motor neuron progenitors will grow as aggregated clumps as they proliferate and expand at least 3 fold. Images provided by Lucia Marani and Prof. Mark Lewis (Loughborough University).
Axol’s Human iPSC-Derived Motor Neuron Progenitors express cell fate markers.
Protein expression of OLIG2, nestin and SOX1 was confirmed in Axol iPSC-Derived Motor Neuron Progenitors using immunocytochemistry. iPSC-Motor Neuron Progenitors were cultured in motor neuron recovery medium (ax0071) for 3 days after thawing before staining for OLIG2, nestin and SOX1. nestin and SOX1 staining (red) confirmed early neural ectoderm marker and OLIG2 staining (red) confirmed motor neuron progenitor differentiation. Images were counterstained with Hoechst.
Axol’s human iPSC-derived motor neurons show the expression of HB9, LIM3 and ChAT, which are typically expressed in mature motor neurons.
Protein expression of HB9, LIM3 and ChAT was confirmed in Axol iPSC-derived motor neuron using immunocytochemistry. Human iPSC-motor neurons were cultured in motor neuron maintenance medium (ax0072) for 14 days post passaging before staining for motor neuron markers LIM3 (green), HB9 (red) and ChAT (red) along with staining for neural markers TUJ1 (green) and MAP2 (red). Merged images were counterstained with Hoechst.